A Phase 1/2 Open Label Study Evaluating the Safety and Efficacy of Gene Therapy of the Sickle Cell Disease by Transplantation of an Autologous CD34+ Enriched Cell Fraction That Contains CD34+ Cells Transduced ex Vivo With the GLOBE1 Lentiviral Vector Expressing the βAS3 Globin Gene (GLOBE1 βAS3 Modified Autologous CD34+ Cells) in Patients With Sickle Cell Disease (SCD)

About the study

The purpose of this study is to evaluate the Safety and Efficacy of Gene Therapy of the
Sickle Cell disease by Transplantation of an Autologous CD34+ enriched cell fraction that
contains CD34+ cells transduced ex vivo with the GLOBE1 lentiviral vector expressing the βAS3
globin gene (GLOBE1 βAS3 Modified Autologous CD34+ Cells) in Patients with Sickle Cell
Disease (SCD)

Study point of contact

Valérie JOLAINE, Manager
+33 1 42 19 28 79
[email protected]
Marina CAVAZZANA, MD & PhD
+33 144495068
[email protected]

Locations

1 France site

Age

12 to 20 Years

Genotypes

HbSS

Phase

Phase 1/Phase 2

Study type

Interventional

Gender

All

Interventions

Genetic

Compensation

Unknown

participation requirements

– – Age 12-20 years

– Diagnosis of HbSS or S-beta zero thalassemia by Hb electrophoresis or genetic
analysis.

– Clinical history or ongoing evidence of severe sickle cell anemia with one OR more of
the following clinical complications demonstrating disease severity:

– At least 3 vaso occlusive crises requiring hospitalization, under hydroxyurea or
transfusion, within 2 years prior to enrollment

– One severe acute chest syndrome (ACS) hospitalized in intensive care unit

– At least 2 episodes of ACS within the prior 3 years), including one under HU.

– Acute priapism (at least 2 episodes > 3h in the preceding year or in the year
prior to the start of a regular transfusion program), OR stuttering priapism ≥ 1
by week under sickle cell treatment (HU, transfusion or phlebotomy).

– Cerebral vasculopathy confirmed by MRA (magnetic resonance angiography) without
Moya-moya

– Presence of sickle cell cardiomyopathy documented by Doppler echocardiography
(left ventricular ejection fraction (LVEF) <55% AND tricuspid regurgitation velocity >2.5m/s on cardiac echocardiograph),

– Tricuspid regurgitation velocity >2.8m/s on cardiac echocardiograph without
pulmonary hypertension confirmed by right heart catheterization (mPAP<25mmHg) - Failed hydroxyurea (HU) therapy, were unable to tolerate HU therapy, or, if 18 years of age or older, have actively made the choice to not take the recommended daily HU regimen. Inadequate clinical response to HU, defined as any one of the following outcomes, while on HU for at least 3 months: 2 or more acute sickle pain crises requiring hospitalization, no rise in Hb >1.5 gm/dl from pre-HU baseline or requires
transfusion to maintain Hb > 6.0 gm/dL, had an episode of ACS despite adequate
supportive care measures.

– Karnovsky/Lansky performance score ≥ 60%

– Sexually active patients must be willing to use an acceptable method of double-barrier
contraception for at least 12 months post-infusion (beyond 12 months at the discretion
of the investigator)

participation restrictions

– Chromosomal (karyotyping) or molecular anomalies (detected by NGS) ( ie 7 chromosomal
monosomy)

– Existence of a matched sibling donor

– Patients who have started new treatment for SCD within 6months of enrollment

– Hematologic evaluation: Leukopenia (WBC < 3000 µL) ( en cours) or neutropenia (ANC < 1000 µL) or thrombocytopenia (platelet count < 100,000 µL) (not due to an erythropheresis procedure) - PT/INR or PTT > 1.5 times upper limit of normal (ULN) or clinically significant
bleeding disorder

– Evaluations within 6 months prior to screening visit:

– ALT or AST > 3 times ULN

– Liver Cirrhosis suspicion on echography, CT scan or MRI AND confirmed by histology

– Cardiac evaluation: LVEF < 40% by cardiac echocardiogram or by MUGA scan - Stroke with significant CNS sequelae i.e., Rankin > 2

– Lung interstitial infiltrate AND Forced Vital Capacity less than 70% AND DLCO less
than 60% at steady state

– Confirmed pulmonary hypertension defined by a right heart catheterization
(PAPm>25mmHg). Right heart catheterization is required if tricuspid regurgitation
velocity >2.8m/s on cardiac echocardiograph OR >2.5m/s with an abnormal Brain
Natriuretic Peptide dosage or an important decrease in transcutaneous Hb O2 saturation
during the 6 minutes walk test.

– Seropositivity for HIV (Human Immunodeficiency Virus), HCV (Hepatitis C Virus), HTLV-1
(Human T-Lymphotropic Virus), or active Hepatitis B Virus, or active infection by CMV
or parvovirus B19, based on positive blood PCR.

– Pregnancy or breastfeeding in a postpartum female

– Any current cancer or prior history of a malignant disease, with the exception of
curatively treated non-melanoma skin cancer

– Immediate family member with an established or suspected Familial Cancer Syndrome

– Diagnosis of significant psychiatric disorder of the subject that could seriously
impeded the ability to participate in the study

– Patients who failed previous HSCT and are severely ill

– Any clinically significant active infection

– Participation in another clinical study with an investigational drug within 30 days of
screening

– Any condition, based on perspective of the medical monitor and treating investigator,
which may lead to increased safety risk or inability to comply with the protocol

Locations

  • Paris, France, Department of Biotherapy, Necker-Enfants Malades Hospital, 75015 [Recruiting]
Last updated 2021-08-04